Branch
Imaging
5 groups13 techniques13 virtual labs
0 of 13 practised
- Light microscopy5 techniques
- Brightfield & phase contrastCheck cell morphology and confluence during routine culture.
- Widefield fluorescenceVisualize fluorescent proteins or dyes in cells.
- Confocal microscopyTake optical sections that reject out-of-focus light and build 3D stacks.
- Light-sheet microscopyImage cleared tissues or live embryos quickly with little photodamage.
- Super-resolutionResolve structures below the ~200 nm diffraction limit.
0 of 5 practisedOpen Light microscopy → - Labelling2 techniques
- ImmunofluorescenceLocalize proteins inside cells with labelled antibodies.
- Fluorescent proteins & dyesTag proteins, organelles or DNA in cells.
0 of 2 practisedOpen Labelling → - Dynamic imaging3 techniques
- Live-cell & time-lapseFollow division, migration and other processes over time.
- FRET, FRAP & FLIMProbe molecular proximity, mobility or local environment.
- Calcium imagingRecord cell activity through calcium-indicator fluorescence.
0 of 3 practisedOpen Dynamic imaging → - Electron microscopy1 techniques0 of 1 practisedOpen Electron microscopy →
- Documentation & analysis2 techniques
- Gel & blot imagingCapture and quantify gels and blots.
- Image analysisMeasure intensity, cell counts and co-localization from images.
0 of 2 practisedOpen Documentation & analysis →