Core path · 4 steps
Image cells
Label structures in cells, image them well and turn the pictures into numbers.
0 of 4 steps practisedStart with step 1: Immunofluorescence →
- ImmunofluorescenceNot started
Label the protein you care about with a fluorescent antibody.
- Widefield fluorescenceNot started
Image with the right filters and exposure, without saturating the camera.
- Confocal microscopyNot started
Cut out-of-focus light for sharp optical sections in thick samples.
- Image analysisNot started
Measure intensity, count and colocalise without fooling yourself.