Advanced path · 6 steps
Make and purify a protein
Produce a recombinant protein, purify it and check that what you have is pure, folded and not aggregated.
0 of 6 steps practisedStart with step 1: Recombinant expression →
- Recombinant expressionNot started
Choose a host and conditions that give soluble protein.
- Affinity purificationNot started
Pull the tagged protein out of the lysate in a single step.
- Chromatography polishingNot started
Remove the remaining contaminants and aggregates by size or charge.
- Thermal shift assaysNot started
Check the protein is folded and find the buffer that keeps it most stable.