BenchMasteryMolecular biology techniques & virtual lab
Core path · 5 steps

Measure gene expression

Find out how much a gene is switched on: from isolating RNA to a fold change you can defend.

0 of 5 steps practisedStart with step 1: RNA extraction →
  1. RNA extractionNot started

    Isolate RNA without letting RNases or genomic DNA spoil it.

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  2. RNA integrity checkNot started

    Check the RNA is intact; degraded RNA distorts every measurement after it.

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  3. Reverse transcriptionNot started

    Copy the RNA into cDNA, the template qPCR can read.

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  4. RT-qPCRNot started

    Quantify the transcript against reference genes and calculate the fold change.

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  5. RNA-seqNot started

    Scale up from a few genes to the whole transcriptome when you need the full picture.

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