Advanced path · 6 steps
Sequence a genome
Take DNA from extraction to reads you can analyse: the next-generation sequencing workflow.
0 of 6 steps practisedStart with step 1: Genomic DNA extraction →
- Genomic DNA extractionNot started
Isolate long, clean DNA, the starting material for everything after.
- DNA quantification & QCNot started
Measure DNA amount and purity, because library prep is sensitive to both.
- Library constructionNot started
Fragment the DNA and add the adapters and indexes the sequencer needs.
- Library QC & poolingNot started
Check library size and concentration, then pool samples evenly.
- Short-read sequencingNot started
Run the sequencer and judge the run from its quality metrics.
- Read processing & analysisNot started
Trim, align and call variants to turn reads into results.