Branch
Cell culture
4 groups18 techniques18 virtual labs
0 of 18 practised
- Maintenance5 techniques
- Aseptic technique & passagingMaintain cell lines in a biosafety cabinet and split them before confluence.
- Media & supplementsChoose and prepare media that keep a cell type healthy.
- Cell counting & viabilityCount cells and check viability to seed at a known density.
- Cryopreservation & bankingFreeze cells in DMSO-containing medium and store them long term.
- Contamination controlCatch mycoplasma, microbial contamination and misidentified lines.
0 of 5 practisedOpen Maintenance → - Culture systems4 techniques
- Primary cell isolationIsolate cells directly from tissue or blood.
- Stem cell cultureGrow and differentiate embryonic, induced pluripotent or adult stem cells.
- 3D culture & organoidsGrow cells in structures that model real tissue more closely than a monolayer.
- Co-cultureGrow two or more cell types together to study how they interact.
0 of 4 practisedOpen Culture systems → - Gene delivery3 techniques
- TransfectionDeliver DNA or RNA into cells for transient expression.
- Viral transductionDeliver genes efficiently, including into primary cells.
- Stable cell line generationSelect and expand clones that permanently carry a construct.
0 of 3 practisedOpen Gene delivery → - Cell-based assays6 techniques
- Viability & cytotoxicityMeasure how a treatment affects cell survival.
- Proliferation & cell cycleMeasure how fast cells divide and where they sit in the cycle.
- Apoptosis assaysDetect programmed cell death.
- Reporter assaysRead out promoter or pathway activity through a reporter gene.
- Migration & invasionMeasure how cells move or invade through a barrier.
- Flow cytometry & FACSMeasure markers on thousands of single cells and sort the populations you want.
0 of 6 practisedOpen Cell-based assays →