Aseptic technique & passagingpassage no.
Maintain cell lines in a biosafety cabinet and split them before confluence.
Sub-techniques
Cell culture
Maintenance
5 techniques5 virtual labs
0 of 5 practised
Media & supplementsformulation
Choose and prepare media that keep a cell type healthy.
Sub-techniques
Cell counting & viabilitycells/mL · % viable
Count cells and check viability to seed at a known density.
Sub-techniques
Cryopreservation & bankingcell bank
Freeze cells in DMSO-containing medium and store them long term.
Contamination controlpos / neg
Catch mycoplasma, microbial contamination and misidentified lines.
Sub-techniques