Branch
DNA
5 groups20 techniques20 virtual labs
0 of 20 practised
- Isolation & QC3 techniques
- Genomic DNA extractionPurify DNA from cells, tissue, blood or plants.
- Plasmid preparationPurify plasmid DNA from bacterial culture.
- DNA quantification & QCMeasure concentration and purity before downstream steps.
0 of 3 practisedOpen Isolation & QC → - Amplification4 techniques
- PCRAmplify a specific DNA region from a small amount of template.
- Quantitative PCR (qPCR)Measure how much of a DNA target is present, in real time.
- Digital PCRCount target molecules absolutely by splitting the reaction into thousands of partitions.
- Isothermal amplificationAmplify DNA at one temperature, often for field or point-of-care tests.
0 of 4 practisedOpen Amplification → - Analysis & detection4 techniques
- Agarose gel electrophoresisSeparate DNA fragments by size to check PCR products, digests and integrity.
- Southern blotDetect a specific sequence in genomic DNA with a labelled probe; largely replaced by PCR.
- GenotypingDetermine which alleles a sample carries at known positions.
- DNA methylation analysisMeasure cytosine methylation at specific sites or genome-wide.
0 of 4 practisedOpen Analysis & detection → - Cloning & assembly5 techniques
- Restriction digestionCut DNA at defined recognition sites for cloning or diagnostic mapping.
- Ligation-based cloningJoin an insert into a cut vector with DNA ligase.
- Seamless assemblyJoin several fragments in one reaction without leftover scar sequences.
- Bacterial transformationIntroduce plasmids into competent E. coli to propagate them.
- Site-directed mutagenesisIntroduce a defined point mutation, insertion or deletion into a plasmid.
0 of 5 practisedOpen Cloning & assembly → - Genome editing4 techniques
- CRISPR-Cas9 editingKnock out, knock in or correct a gene at a chosen locus.
- Base & prime editingMake precise single-base or small edits without a double-strand break.
- CRISPR screensTest thousands of gene perturbations at once to find hits for a phenotype.
- Other editing & transgenesisOlder or complementary ways to modify genomes.
0 of 4 practisedOpen Genome editing →