Restriction digestionfragment sizes
Cut DNA at defined recognition sites for cloning or diagnostic mapping.
Sub-techniques
DNA
Cloning & assembly
5 techniques5 virtual labs
0 of 5 practised
Ligation-based cloningcolonies
Join an insert into a cut vector with DNA ligase.
Sub-techniques
Seamless assemblycolonies
Join several fragments in one reaction without leftover scar sequences.
Sub-techniques
Bacterial transformationcolonies
Introduce plasmids into competent E. coli to propagate them.
Sub-techniques
Site-directed mutagenesisverified clone
Introduce a defined point mutation, insertion or deletion into a plasmid.
Sub-techniques