Isothermal titration calorimetryKD · ΔH · n
Measure affinity, stoichiometry and binding thermodynamics directly.
Structure & biophysics
Binding & interactions
5 techniques5 virtual labs
0 of 5 practised
SPR & BLIKD · kon · koff
Measure real-time binding kinetics to an immobilized partner.
Measure binding in solution using very little sample.
Detect a small labelled ligand binding to a larger partner.
EMSA (gel shift)shifted band
Show that a protein binds a DNA or RNA probe.